Part:BBa_K2905000

MttB

Trimethylamine methyltransferase. Requires pyrrolysine to be added to stop codon within the open reading frame.

Starts with a His-6 tag, followed by a NheI site scar and a TEV protease recognition site, fused in frame to the MttB gene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 43
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1159
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 808
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 985